Salmonella and E. coli experiments

Overview

Created: 2019-02-05. Parsed: 2019-03-08

Aim of the project

Study:

• interaction of curli-producing bacteria (selected from the collection of strains using the computational model) with mapranosis-related cell lines,
• regulation of biofilm formation

Methodology

For the experiment we have used collection of Salmonella (\(n \approx 50\)) and Escherichia (\(n \approx 250\)) strains.

Salmonella growth conditions:

• Temperature: 28, 37
• Time: 48h
• Type of Plate: polystyrene, polypropylene

Escherichia coli growth conditions:

• Temperature: 37
• Time: 48h
• Type of Plate: polystyrene

Salmonella | Results as boxplot

Salmonella | Average fluorescence intensity

Average intensity of all repeats.

Salmonella | Biofilm forming strength

Salmonella | Biofilm forming strength - consensus strains

All repeats in specific conditions match.

Salmonella | Biofilm forming strength - consensus strains | Table

E. coli | All results as boxplot

E. coli | All results showed as boxplot, split 1/2

E. coli | All results showed as boxplot, split 2/2

E. coli | Average fluorescence intensity

Average intensity of all repeats.

E. coli | Average fluorescence intensity, split 1/2

E. coli | Average fluorescence intensity, split 2/2

E. coli | Biofilm forming strength

Strains 4516, 5084, 5093, 5096, 5541 do not have second repeat due to file corruption (software saved intensiy values as dates).

E. coli | Biofilm forming strength, split 1/2

E. coli | Biofilm forming strength, split 2/2

E. coli | Biofilm forming strength - consensus strains

All repeats in specific conditions match.

E. coli | Biofilm forming strength - consensus strains | Table

Absence biofilm formers

There are some Salmonella strains that in every condition do not produce any biofilm.

Weak biofilm formers

There are no strains that in every condition produce very weak biofilm.

Medium biofilm formers

There are no strains that in every condition produce very weak biofilm.

Strong biofilm formers

There are E. coli strains that in every condition produce very strong biofilm.

Conclusion

• We have found that some of Salmonella strains do not produce any biofilm in every conditions that we have used.

• Some E. coli strains form strong biofilms in every conditions.

• We have not found a single strain of Salmonella or E. coli that form weak or medium biofilm in conditions we have used.

• E. coli 6101 strain have the bigest intensity variance.

• All E. coli strains produce biofilms.

Salmonella | Average fluorescence intensity | mean = replicates + repeats

Average intensity of all repeats.

Salmonella | Average fluorescence intensity | mean = replicates + repeats + median

Average intensity of all repeats.

Salmonella | Biofilm forming strength | mean = replicates + repeats

Salmonella | Biofilm forming strength | mean = replicates + repeats + medium

E. coli | Average fluorescence intensity | mean = replicates + repeats

Average intensity of all repeats.

E. coli | Average fluorescence intensity, split 1/2 | mean = replicates + repeats

E. coli | Average fluorescence intensity, split 2/2 | mean = replicates + repeats

E. coli | Average fluorescence intensity | mean = replicates + repeats + medium

Average intensity of all repeats.

E. coli | Average fluorescence intensity, split 1/2 | mean = replicates + repeats + medium

E. coli | Average fluorescence intensity, split 2/2 | mean = replicates + repeats + medium

E. coli | Biofilm forming strength | mean = replicates + repeats

Strains 4516, 5084, 5093, 5096, 5541 do not have second repeat due to file corruption (software saved intensiy values as dates).

E. coli | Biofilm forming strength, split 1/2 | mean = replicates + repeats

E. coli | Biofilm forming strength, split 2/2 | mean = replicates + repeats

E. coli | Biofilm forming strength | mean = replicates + repeats + medium

Strains 4516, 5084, 5093, 5096, 5541 do not have second repeat due to file corruption (software saved intensiy values as dates).

E. coli | Biofilm forming strength, split 1/2 | mean = replicates + repeats + medium

E. coli | Biofilm forming strength, split 2/2 | mean = replicates + repeats + medium